Updated documentation – January 2015
The viral disease IPN has traditionally been a problem for rainbow trout in the fresh water phase. The disease may cause major losses, both in increased mortality rates, reduced growth and instances of weakened fish that have survived an IPN outbreak. This means a high degree of uncertainty as to how fish will perform compared to annual production budgets.
In 2009 AquaGen performed the first selection for IPN resistance in rainbow trout using traditional family based breeding. In parallel with this work, a number of challenge tests were used to identify and document genetic markers that can be used to select broodstock with IPN resistant genes. It was proved that IPN resistance in rainbow trout is to a large extent controlled by one single QTL, just like in salmon. The first eggs selected for this QTL were delivered to fish farmers in January 2015.
Effect testing of IPN resistance
The effect of the genetic marker has been documented in two separate challenge tests on rainbow trout fry soon after start feeding. In order to eliminate environmental effects between test groups all fish were challenged in the same tank. At the end of the experiments both living and dead fish were genotyped and grouped as respectively qq (homozygote, IPN sensitive), qQ (heterozygote) and QQ (homozygote, IPN strong).
The results from both experiments show a significant difference in mortality rates between the different genotypes, where the degree of IPN resistance depends on the frequency of Q (see Figure 1 and 2).
In experiment 1 the frequency of the favorable IPN resistant variant Q was relatively low (25%). This has contributed to the building up of a high IPN infection pressure because of the high number of IPN sensitive fish (qq) in the group. In experiment 2 the genotypes were more evenly distributed (48% Q), and had a moderate IPN infection pressure. In both tests the mortality rate was considerably lower in the groups that carried the IPN resistant variant Q, and the difference was especially high when the infection pressure was moderate. These infection experiments document that the QTL for IPN resistance in rainbow trout has a good effect on IPN in laboratory experiments.
Great expectations for protection by QTL-selection
In the eggs selected for IPN resistance (that only have the genotypes qQ and QQ), the frequency of Q will be 60–80%. This level of frequency will provide a positive population effect (a kind of “herd immunity”) that means there will be a higher threshold for the IPN virus to overcome to establish itself in the group of fish, and the extent of a potential IPN outbreak will be considerably reduced.
Products with IPN-resistance:
- AquaGen® Rainbow QTL-innOva® IPN
Advantages of Rainbow QTL-innOva® IPN:
- IPN protection through the entire life of the fish
- Greater predictability in production of rainbow trout